Combi Flash 7650 Series Manual do Utilizador descarregar pdf (Página 77)

Section 3 Operation

3-15

3.5 During the Run

You can monitor the progress of the run on the PeakTrak main

window. If desired, you can modify gradient parameters

during the run. (See the Real-time Gradient Editing on-line

help topic.)

As the run progresses, the absorbance trace is drawn on the

Gradient Plot area. The separation or purification continues

until the end of the run defined by the run length setting.

During the run, data is automatically saved every few seconds.

PeakTrak automatically saves all run history on the system’s

internal hard drive in its native .RUN format. You can

manually save a Run file as Text or PDF while stopped or any

later time as long as the .RUN file has not been deleted.

Selected files can be manually saved to a connected Flash

drive. To automatically save a Text or PDF file after each run,

configure the Network File Save feature (see Section 5.1.3).

3.5.1 Run Control Buttons

• Play — The Play button starts the run. During the run,

other run control buttons are active.

• Pause — The Pause button holds the %B at the current

value while the system continues to operate (sometimes

called an isocratic hold). Note that pausing the run

extends the run length. While in the Paused state, you

can resume the run by clicking the Play button, or stop

the run by clicking the Stop button. If you resume the

run, the system continues with the gradient curve at the

%B when the system was paused.

• Stop — The Stop button suspends the entire run.

Unlike the Paused state, the pump, peak detection, and

fraction collection will not operate. While stopped, you

can end the run by clicking the Rewind button, resume

the run by clicking the Play button, or skip the rest of

the run sequence by clicking the Fast Forward button.

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Combi Flash 7650 Series Manual do Utilizador Página 77