Combi Flash 7650 Series Manual do Utilizador

iCombiFlash Tor re n t®User ManualFor indoor use onlyCOPYRIGHT © 2009 byTeledyne Isco4700 Superior St., Lincoln, Nebraska, U.S.A. 68504Phone: (402) 46

CombiFlash Torrent® User Manual1-41.4 SpecificationsTable 1-1 CombiFlash Torrent Module Specifications1Sample size: 0.5 to 300 gramsRediSep Rf column

CombiFlash Torrent® User Manual4-204.3.2.6 Peak DetectionThis section of the window contains option buttons to enable and disable various peak detect

Section 4 User Help Reference4-21Note: If both Slope and Threshold peak detection methods are checked, PeakTrak considers a peak to be present when a

CombiFlash Torrent® User Manual4-22To open the Gradient Optimizer window, open the METHOD EDITOR [4.3.2] window, then click the Gradient Optimizer but

Section 4 User Help Reference4-23command to prevent the original file from being overwritten.• Delete — Click this button to delete a highlighted fil

CombiFlash Torrent® User Manual4-244.3.6 Set Data PathThe FILE>SET DATA PATH… [4.2.1.4] menu command opens the Set Data Path window. Use this wind

Section 4 User Help Reference4-25• Progress bar — This bar displays how much of the solvent has been pumped. • Play button — Click this button to sta

CombiFlash Torrent® User Manual4-26• Flow Rate – Type or select the pump’s flow rate in mL/min.• Pump into Tube # – As a default setting, solvent is p

Section 4 User Help Reference4-27by selecting the TOOLS>MANUAL CONTROL [4.2.3.3] menu command.The configuration window displays four tabs: INSTRUM

CombiFlash Torrent® User Manual4-28• Enable solvent B priming pump — The CombiFlash Torrent module is equipped with a priming pump for the B solvent t

Section 4 User Help Reference4-29volume. During a run, other factors such as automatic tube advances for detected peaks will affect collected volume.

Section 1 Introduction1-51.5 Controls, Indicators, and FeaturesFigures 1-1 through 1-3 illustrate key features on the Torrent.Figure 1-1 CombiFlash

CombiFlash Torrent® User Manual4-30Follow the on-screen instructions for entering the address and queue information. After successfully printing a tes

Section 4 User Help Reference4-31the programmed run length. This ensures that a late-eluting peak is still collected.Gradient Method Select the desir

CombiFlash Torrent® User Manual4-32A delay volume must also be specified for each Foxy fraction collector connected as a system. If using the Fraction

Section 4 User Help Reference4-333.To protect the Default methods select the “Require Password For Editing Default Methods” option.4.Click the OK but

CombiFlash Torrent® User Manual4-34•MONITORING THE WAVELENGTH RATIO [4.4.7]•BYPASSING THE SOLID LOAD CARTRIDGE [4.4.8]•VIEWING RUNS [4.4.9]•MANUAL CON

Section 4 User Help Reference4-35equilibration and then wait while you prepare the cartridge. After you have placed the cartridge on the module, clic

CombiFlash Torrent® User Manual4-36If you selected Solid (Pause), the module equilibrates the column with the starting %B and then waits while you pre

Section 4 User Help Reference4-37If you will transfer the sample mixture directly from a container, attach the tubing to the pump inlet. Next, place

CombiFlash Torrent® User Manual4-384.4.2.1 Basic method settingsThe basic settings allow you to control the %B gradient mix, flow rate, solvents, and

Section 4 User Help Reference4-39• RediSep Rf 3 kg = 4.8 LOther media types will have similar column volumes. Refer to the REDISEP RF COLUMN SELECTIO

CombiFlash Torrent® User Manual1-61. Touch Panel LCD display – Large 10.4 inch display for system monitoring and control.2. On/Standby Switch – 1 = On

CombiFlash Torrent® User Manual4-40When using the All or Peaks options, you can specify the maximum Peak and Non-peak volumes to be collected in the t

Section 4 User Help Reference4-41• Peak Width — Select the average peak width setting. Peak widths are measured at the baseline. The slope detector w

CombiFlash Torrent® User Manual4-42the External Detector instruction sheet for electrical and plumbing requirements.• Show Ratio — If using two of the

Section 4 User Help Reference4-43save the method using the FILE>Save or Save As menu commands.4.4.3.3 Scaling Method Files to Different Column Si

CombiFlash Torrent® User Manual4-44button. The settings will revert to the original factory settings. Click the Save button to save the settings for f

Section 4 User Help Reference4-454.4.7 Monitoring the Wavelength RatioWhen using two peak detection options, such as 1 with 2 or All Wavelength De

CombiFlash Torrent® User Manual4-46CAUTION: Bypassing the solid load cartridge too soon during the purification run may leave desired compounds on the

Section 4 User Help Reference4-47TLC, wait at least the time established as T. After this time has elapsed it is likely that the desired compound has

CombiFlash Torrent® User Manual4-48• Chromatogram — The right side of the window displays the chromatogram, identified by the Sample Name in the title

Section 4 User Help Reference4-49• Close button — Click the Close button when you are done viewing the run.4.4.9.1 General instructions for reading

Section 1 Introduction1-7media of choice. The cartridge outlet connects to the sample injection port (9).12.Solid Load Cartridge Cap – Adds the cartr

CombiFlash Torrent® User Manual4-50module, first open the MANUAL CONTROL [4.3.9] window by selecting the Tools>Manual Control menu command.4.4.10.1

Section 4 User Help Reference4-51for modules with flow cell windows stained by compounds.• Red – lamp energy is obstructed to a degree that the modul

CombiFlash Torrent® User Manual4-52ambient environment of your laboratory. In this case, increase the setting of the Vapor Limit. Settings greater tha

5-1CombiFlash To r re n t®User ManualSection 5 Remote InterfacesThis section provides instructions for controlling the CombiFlash Torrent module throu

CombiFlash Torrent® User Manual5-26. Confirm that your network administrator has completed any necessary network changes to support the system. 7. Usi

Section 5 Remote Interfaces5-3uses a JetDirect print queue, also ask for the port number. When this information is known, you can proceed with config

CombiFlash Torrent® User Manual5-4(iPod Touch, iPhone, and iPad1) running iOS 4.2 or later. The PeakTrak app is available for download from the App St

Section 5 Remote Interfaces5-5NoteThe following instructions may require a user account with Administrator privileges on the PC. If you cannot modify

CombiFlash Torrent® User Manual5-65.2.2 Windows Vista Settings1. From the Windows Start menu, right-click on Network, then select the Properties menu

Section 5 Remote Interfaces5-7Figure 5-2 Internet Protocol Version 4 (TCP/IPv4) PropertiesNoteIf the Internet Protocol Version 4 (TCP/IPv4) option i

CombiFlash Torrent® User Manual1-816.Gas Inlet – The Torrent has an internal air pump that delivers air through the column for a post-run purge. The a

CombiFlash Torrent® User Manual5-85.2.3 Windows XP Settings1. From the Windows Start button, open the Control Panel.2. Locate the Network Connection

Section 5 Remote Interfaces5-9Figure 5-4 Local Area Connection PropertiesFigure 5-5 Alternate Configuration Settings

CombiFlash Torrent® User Manual5-105.2.4 Windows 2000 Settings1. From the Windows Start button, select “Settings>Control Panel” to view the contro

Section 5 Remote Interfaces5-11Figure 5-6 Local Area Connection Properties WindowFigure 5-7 Internet Protocol Properties Window

CombiFlash Torrent® User Manual5-125.2.5 Completing the Direct Connection1. Connect the PC to the Torrent using the cross-over cable.2. Start Interne

6-1CombiFlash To r re n t®User ManualSection 6 Maintenance6.1 System Standby and Shut Down During extended periods of inactivity, you can place the s

CombiFlash Torrent® User Manual6-26.2 Preventive MaintenanceThe system requires preventive maintenance by the user for safe and reliable operation. R

Section 6 Maintenance6-36.3 CleaningTo clean the exterior surfaces, use a cleaning cloth dampened with a mixture of distilled water and a mild deter

CombiFlash Torrent® User Manual6-46.4 Tubing InspectionWARNINGRisk of fire or equipment damage. Faulty tubing, fittings, and drains may allow organic

Section 6 Maintenance6-5Figure 6-1 Default post-separation column and flow cell flushFigure 6-2 Flow cell cleaning messageTypically, chemists Stop

Section 1 Introduction1-9Figure 1-3 CombiFlash Torrent Features (Back)29.Cooling Fan – Cools the internal components.30.Ethernet Port – An 8P8C jack

CombiFlash Torrent® User Manual6-6CAUTIONWhen using methanol as solvent B with silica column media, do not pump more than 50% solvent B. Higher percen

Section 6 Maintenance6-7• Red – lamp energy is obstructed to a degree that the system might not reliably detect peaks. If you attempt to operate the

CombiFlash Torrent® User Manual6-8NotePumping solvent B at a moderate flow rate (150 to 200 mL/min) over time will usually solubilize obstructions. Ge

Section 6 Maintenance6-9Table 6-1 Suggested Flow Cell Cleaning SolventsaSolvent DescriptionMethanol Miscible with most LC solvents. Methanol will for

CombiFlash Torrent® User Manual6-106.5.3 Monthly Flow Cell CleaningPerform this procedure as part of your scheduled preventive maintenance, or when Q

Section 6 Maintenance6-116.6 Electrical TroubleshootingIf your instrument stops working and the touch panel display is off, check the line cord conn

CombiFlash Torrent® User Manual6-12

DECLARATION OF CONFORMITY Application of Council Directive: 2004/108/EC -The EMC Directive 2002/96/EC – The WEEE Directive 73/23/EEC –

CombiFlash Hazmat Table 60-5233-406 Rev. B ℶ❐₼㦘㹡㦘⹂䓸德㒥⏒侯䤓⚜䱿♙⚺摞 Name and amount of Hazardous Substances or Elements in the product 捷ↅ⚜䱿 Compon

CombiFlash Torrent® User Manual1-10WARNINGLiquids associated with this instrument may be classified as carcinogenic, biohazard, flammable, or radioact

Section 1 Introduction1-111.6.1 Hazard Severity LevelsThis manual applies Hazard Severity Levels to the safety alerts. These three levels are descri

CombiFlash Torrent® User Manual1-12Table 1-2 Hazard SymbolsWarnings and CautionsThe exclamation point within the triangle is a warning sign alerting y

Section 1 Introduction1-131.7 For Additional InformationTechnical assistance for the Torrent can be obtained from:Teledyne Isco4700 Superior St.Linc

CombiFlash Torrent® User ManualiiTeledyne Isco One Year Limited Warranty* Factory Service for Teledyne Isco CombiFlash® Systems This warranty exclusiv

CombiFlash Torrent® User Manual1-14

2-1CombiFlash To r re n t®User ManualSection 2 PreparationThis section provides instructions for unpacking and installing the CombiFlash Torrent modul

CombiFlash Torrent® User Manual2-2Compare the contents of the boxes with the enclosed packing slips. If there are any shortages, contact Teledyne Isco

Section 2 Preparation2-3quickly be removed from the outlet in the event of an emergency.CAUTIONUnless power must be removed due to an emergency, alwa

CombiFlash Torrent® User Manual2-42.4.4 Cabling: Network ConnectionThe Torrent can be added to a network or directly connected to a computer for remo

Section 2 Preparation2-5CAUTIONEnsure that the CombiFlash Torrent has at least 3 cm (1.25") of air space behind it for ventilation.Determine whe

CombiFlash Torrent® User Manual2-6NoteIf a System Verification (Section 2.15) will be performed, the required solvents are hexane (Solvent A) and ethy

Section 2 Preparation2-7a. Locate a nut (60-0923-015) and ferrule (60-0083-163) in the accessory package (60-5249-002).b. Insert the ferrule, narrow

CombiFlash Torrent® User Manual2-82.6.3 Pressurized Solvent Lines in the LaboratoryWhen receiving solvent from pressurized lines on-site, the Torrent

Section 2 Preparation2-9CAUTIONElevated flammable vapor levels are possible. Ensure that the waste container is adequately ventilated, preferably by

iiiCombiFlash To r re n t®User ManualTable of Contents1. Introduction1.1 Documentation Overview . . . . . . . . . . . . . . . . . . . . . 1-11.2 Pro

CombiFlash Torrent® User Manual2-10If using the optional Waste Outlet Cap (60-5394-512 for 83B closures, 60-5394-513 for 100 mm closures), proceed wit

Section 2 Preparation2-11If an optional waste cap is not used, ensure the waste lines are secured so they drain into the waste container. Secure the

CombiFlash Torrent® User Manual2-122.10 Install Optional EquipmentThe installation of optional equipment that was electrically connected to the Torre

Section 2 Preparation2-132.10.2 Optional Foxy R2 High Flow Fraction CollectorThe fraction collector(s) should be positioned to the right of the Torr

CombiFlash Torrent® User Manual2-14• Use the supplied cable ties to secure the tubing as desired. Before turning on any fraction collector, manually m

Section 2 Preparation2-15TCP/IP Settings in the Foxy R2The IP address and netmask are entered from the fraction collector front panel.1. Select Confi

CombiFlash Torrent® User Manual2-16TCP/IP Settings in the TorrentThe network information for the Torrent is entered from the front panel.1. Select Too

Section 2 Preparation2-17Torrent/Foxy R2 Network Configuration: ExamplesTypical examples of Torrent/Foxy R2 network connection are provided in this s

CombiFlash Torrent® User Manual2-18In Example 2, the system runs on your network with each unit directly connected to your network.Figure 2-9 Network

Section 2 Preparation2-19In Example 3, the system runs on your network with each unit connected to an Ethernet hub, and then to your network. The pas

CombiFlash Torrent® User Manualiv2.10.3 Optional Foxy R2 High Flow Fraction Collector . . . . . . . . . . . . . . . . . . . . . .2-142.10.4 Model 340

CombiFlash Torrent® User Manual2-202.10.3 Optional Fractionation ValveThe fractionation valve is an alternative to the typical Foxy fraction collecto

Section 2 Preparation2-212. Connect the supplied tubing between the Torrent’s TO COLLECTOR port and the Fractionation Valve’s diverter valve IN port

CombiFlash Torrent® User Manual2-22Figure 2-14 Six-port valve connections123456Collection VesselsFromTorrentNormallyClosedCommonNormallyOpenWasteFrom

Section 2 Preparation2-232.10.4 Model 340CF Evaporative Light Scattering Detector (ELSD)The Torrent can be configured for use with Teledyne Isco’s 3

CombiFlash Torrent® User Manual2-24Plumbing the Torrent to the ELSD requires the connection package, part #60-5247-021. The package contains the follo

Section 2 Preparation2-252. Using the two long screws with lock and flat washers, mount the metal tee fitting on the rear panel, aligning the two sta

CombiFlash Torrent® User Manual2-26Figure 2-18 Torrent: Disconnect tubing from bottom column connection5. Locate the black PTFE tubing that is swaged

Section 2 Preparation2-27Figure 2-19 Tee fitting for Torrent to ELSD2.10.5 User-supplied External DetectorThe Torrent can be configured for use wit

CombiFlash Torrent® User Manual2-282.11 GroundingWARNINGRisk of fire or explosion. Nonconductive, nonpolar liquids at linear velocities greater than

Section 2 Preparation2-29(sections 2.13.1 through 2.13.3) for the desired operation and then click OK to save the settings.Some configuration setting

Table of Contentsv4.2 PeakTrak Menu Options . . . . . . . . . . . . . . . . . . . . . . 4-34.2.1 File . . . . . . . . . . . . . . . . . . . . . . .

CombiFlash Torrent® User Manual2-30level sensing feature (recommended). Otherwise, uncheck this option to disable this feature. Solvent B Priming Pump

Section 2 Preparation2-31• Ensure that no open containers or spills of organic solvent are in close proximity to the Torrent.• Ensure that the Torren

CombiFlash Torrent® User Manual2-32Automatically Print Report at End of Run – if this option is enabled, the system will print a report at the end of

Section 2 Preparation2-33NoteThe System Verification procedure (section 2.15) requires a Linear gradient method.Enable Run Length Extension – When en

CombiFlash Torrent® User Manual2-34third extensions. At 24 minutes, the %B solvent strength returned to zero. Before the final minute elapsed, more co

Section 2 Preparation2-35Foxy 3 Delay Volume – 60 mLFoxy 4 Delay Volume – 80 mLFractionation Valve Delay Volume – 10 mLIf using custom-length tubing,

CombiFlash Torrent® User Manual2-36CAUTIONExcept during an emergency, never remove AC power immediately after placing the Power switch in the Off posi

Section 2 Preparation2-37NoteWhen pumping solvents the first time, inspect the solvent and waste connections to the system. If any leaks are observed

CombiFlash Torrent® User Manual2-38CAUTIONPinch point. Your fingers or hands can be seriously injured if you place them between the moving parts of th

Section 2 Preparation2-3910.After equilibration, the Torrent will pause for sample injection. Draw 2 mL of the dissolved Test Mix B solution into a s

CombiFlash Torrent® User Manualvi6. Maintenance6.1 System Standby and Shut Down . . . . . . . . . . . . . . . 6-16.2 Preventive Maintenance . . . . .

CombiFlash Torrent® User Manual2-40Figure 2-22 Test Mix B Chromatogram: Peak 1=benzophenone; Peak 2=methyl 4-hydroxybenzoate; Peak 3=4-aminobenzoic a

Section 2 Preparation2-41Table 2-1 Installation Qualification ChecklistStep DescriptionInstaller InitialsOperator Initials2.1UNPACKING THE UNIT2.2IN

CombiFlash Torrent® User Manual2-42

3-1CombiFlash To r re n t®User ManualSection 3 OperationThis section provides abbreviated operating instructions for the CombiFlash Torrent module. Fo

CombiFlash Torrent® User Manual3-2Figure 3-1 Changing column luer adaptersThe cartridge outlet (sample injection port) also has a fitting that can be

Section 3 Operation3-3Figure 3-2 Changing cartridge inlet and outlet adapters 3.1.2 Cartridge Inlet AdapterAn adapter is required when using 25 and

CombiFlash Torrent® User Manual3-4The Direct Injection Adapter is recommended when using the Liquid Load Pump to load samples directly on the column,

Section 3 Operation3-53.2.1 Liquid Sample InjectionIf the sample is soluble in the starting mobile phase, it can be prepared as a solution and injec

CombiFlash Torrent® User Manual3-6c. Agitate the solvent for a moment to allow the sample to adsorb to the silica.d. Remove the solvent with a suitabl

Section 3 Operation3-7Figure 3-3 Press the lever and extend the plunger3. Slide the plunger into the cartridge until it reaches the top frit (Figure

1-1CombiFlash To r re n t®User ManualSection 1 Introduction1.1 Documentation OverviewThis Installation Guide provides:• Safety Information• Unpacking

CombiFlash Torrent® User Manual3-8Figure 3-4 Insert the plunger to the bedFigure 3-5 Align and push cartridge into the cap, and then rotate the cart

Section 3 Operation3-93.3 Loading a RediSep Rf ColumnTo load a RediSep Rf column:WARNINGThe CombiFlash Torrent has redundant safety devices to limit

CombiFlash Torrent® User Manual3-10NoteAfter loading a RediSep Rf column, the system will use RFID technology to automatically detect the media type a

Section 3 Operation3-11cartridge. After you have placed the cartridge on the system, click OK to continue with the run. • Solid – Select this option

CombiFlash Torrent® User Manual3-12•If you selected Solid (Pause), the system equilibrates the column with the starting %B solvent mixture and then wa

Section 3 Operation3-133. Open the valve.4. Inject the liquid sample mixture.5. Close the valve and remove the syringe.NoteChase solvent clears the L

CombiFlash Torrent® User Manual3-14Figure 3-7 Sample Load Pump options2. Prepare the Sample Load pump to receive the sample.If the sample mixture wil

Section 3 Operation3-153.5 During the RunYou can monitor the progress of the run on the PeakTrak main window. If desired, you can modify gradient pa

CombiFlash Torrent® User Manual3-16CAUTIONWhen the Stop button is pressed, solvent remains in the tubing from the injection valve to the detector’s fl

Section 3 Operation3-17NoteWhen you click the Next Tube button, the system immediately marks the tube advance on the chromatogram. However, the syste

CombiFlash Torrent® User Manual1-2The Torrent is optimized for use with Teledyne Isco’s RediSep® Rf columns, which are pre-packed with a variety of me

CombiFlash Torrent® User Manual3-18

4-1CombiFlash To r re n t®User ManualSection 4 User Help ReferenceThe CombiFlash Torrent module includes User Help topics that are available from the

CombiFlash Torrent® User Manual4-2• Module Status — PeakTrak displays the module status to the right of the menu. Status messages may include:• The cu

Section 4 User Help Reference4-34.1.2.1 Default MethodsWhen PeakTrak is started, and every time you insert a RediSep Rf column, the module loads a d

CombiFlash Torrent® User Manual4-4•TOOLS [4.2.3]•HELP [4.2.4]4.2.1 FileThe file menu lists the following commands:4.2.1.1 NewChoose the New command

Section 4 User Help Reference4-54.2.1.5 Save MethodChoose this menu command to save the settings of the current method file to the module’s internal

CombiFlash Torrent® User Manual4-6drive. The file name will be the same as the Run file, except with a “TXT” file name extension. When selecting this

Section 4 User Help Reference4-74.2.1.12 Print…From the touch screen, this command prints the completed run on the network printer (see NETWORK PRIN

CombiFlash Torrent® User Manual4-84.2.3.4 Auto ZeroChoose this command to zero the absorbance trace during a run.4.2.3.5 Edit defaultsChoose this co

Section 4 User Help Reference4-94.2.3.9 Calibrate TouchscreenChoose this command from the touch screen to calibrate the interaction between touch sc

Section 1 Introduction1-31.3 Operating OverviewThe Torrent is equipped with a touch screen display for local control.The system supports TCP/IP comm

CombiFlash Torrent® User Manual4-104.2.4.5 About PeakTrakChoose this command to view information about PeakTrak.4.2.4.6 About IscoChoose this comman

Section 4 User Help Reference4-11the left side of the window changes to show a collection point map or the method parameters in a PEAK COLLECTION DAT

CombiFlash Torrent® User Manual4-12• Sample Name — The sample name is a text entry box in which you can label the run. Type a unique descriptor. PeakT

Section 4 User Help Reference4-13the run data by clicking the Save button, abort the run by clicking the Rewind button, or resume the run by clicking

CombiFlash Torrent® User Manual4-14When an optional fraction collector or fractionation valve is not connected to the system, peak isolation is still

Section 4 User Help Reference4-154.3.2 Method EditorThe Method Editor window has several sections. The BUTTON BAR [4.3.2.1] at the top of this windo

CombiFlash Torrent® User Manual4-16PeakTrak’s default Run Length units by changing the CONFIGURATION [4.3.10] settings.4.3.2.2 Run Settings• RediSep

Section 4 User Help Reference4-17Time Windows can limit the fraction collection to specific time durations of the run. To use time windows, first ent

CombiFlash Torrent® User Manual4-18once for each gradient point that must be added to the plot area.• Delete —Click this button to enable the gradient

Section 4 User Help Reference4-19• Insert Point — This command or button will insert a row below the selected point. • Delete Point — This command wi